PROTEIN EXPRESSION
The Protein Expression Unit is equiped with the range of instruments necessary for E. coli expression system.The instruments include:
- NUAIRE Biological Safety Cabinet Class II
- Scientific 150 litre Shaking Incubator
- FMH Instruments 24 litre water bath
- Jenway 7300 Spectrophotometer
- QSonica 125 Watt Sonicator system
- Biometra SDS-PAGE system
- Electrophoresis and Gel documentation system ( in collaboration with unit 1 )
- Biometra western blotting system
- Dot blot system
- Conventional PCR system ( in collaboration with unit 1)
- His-Tag Purification system ( His Trap)
- Dialysis system
The main service provided at the unit at the moment is production of recombinant proteins using E.coli expression system. Other services include:
- DNA/RNA/Protein extraction and purification ( in collaboration with unit 1 )
- Detection of protein using dot blot and western blot
The unit has enough expertise and technology available to adapt to other purification systems of the proteins with other tags.